14:00
ARRIVALS & REGISTRATION (1st floor)
17:30
CONFERENCE HALL A + B
OPENING CEREMONY
Welcome by Hinke Mulhaupt, the IFSHC president and Klara Weipoltshammer, the SfH president
18:00
CONFERENCE HALL A + B
PLENARY LECTURE | Session Chair: Hinke Mulhaupt
Toyoshi Fujimoto, Juntendo University, Tokyo, Japan
New Roles of Lipid Droplets: A Histochemical Journey
19:00
LORETA I+II
WELCOME RECEPTION
09:00
CONFERENCE HALL A
Structure of the cell, tissues and pathogenesis
Session Chair: Masahiko Harata
09:00‑09:25
Vilmos Péter, Biological Research Centre Szeged, Hungary (INVITED)
Parallel import mechanisms ensure the robust nuclear localization of actin in Drosophila
09:25‑09:50
Masahiko Harata, Tohoku University, Japan
Crosstalks between actin and actin-related proteins in nuclear organization and functions
09:50‑10:05
Martin Sztacho, Institute of Molecular Genetics, Czech Republic
Molecular insight into the mechanism of nuclear PIP2 regulation of RNA Polymerase II transcription
10:05‑10:20
Christian Schöfer, Medical University of Vienna, Austria
Anti-cancer drug CX-5461 causes nucleolar compaction, alteration of peri- and intranucleolar chromatin arrangement, an increase in both heterochromatin and DNA damage response
10:20‑10:45
Ciler Celik Ozenci, Koc University, Turkey (INVITED)
Effects of maternal circadian rhythm disruption on maternal, fetal, placental, and offspring health: Disintegrating early causal pathways of IUGR in a life-course perspective
09:00
CONFERENCE HALL B
Cancer biology
Session Chairs: Karel Smetana, Marco Biggiogera
09:00‑09:25
Nihal Karakas, Istanbul Medipol University, Turkey (INVITED)
Biomarker Studies for the Diagnosis and Therapy of Glioblastoma Brain Tumor
09:25‑09:50
Karel Smetana, First Medical Faculty, Charles University, Czech Republic (INVITED)
Cancer-associated fibroblasts shape the tumour microenvironment and systemic properties of cancer
09:50‑10:05
Mitrajit Ghosh, Nencki Institute of Experimental Biology, PAS, Poland
Defining the microenviroment of murine malignant gliomas with multiplex immunohistochemistry and spatial transcriptomics
10:05‑10:20
Alessia Iaiza, Sapienza, University of Rome, Italy
METTL3 contribution to cell proliferation in Thymic Epithelial Tumor Cells
10:20‑10:35
Phyu Synn Oo, International Medical University, Malaysia
cancelled
Combined effect of Mitochondrial division inhibitor 1 (Mdivi-1) and oestrogen receptor antagonist (Fulvestrant) in ER-positive breast cancer cells
10:35‑10:50
Natalia Glatzel-Plucinska, Wrocław Medical University, Poland
SATB1 protein as a new target for breast cancer immunotherapy with the use of adoptive T cells transfer
10:50
Coffee break (supported by Diatome Ltd.)
11:20
CONFERENCE HALL A + B
PLENARY LECTURE | Session Chair: Klara Weipoltshammer
Robert Feulgen Lecture
Ohad Medalia, University of Zurich, Switzerland
From actin to lamins by in situ structural biology
12:20
Lunch break
13:20
CONFERENCE HALL A + B
PLENARY LECTURE | Session Chair: Zbigniew Kmiec
Bozena Kaminska, Nencki Institute of Experimental Biology, PAS, Poland
Dissecting heterogeneity and roles of brain macrophages in brain tumors: when guardians turn enemies
14:20
COMPANY PRESENTATION | Session Chair: TBA
JEOL
14:50
Coffee break (supported by Diatome Ltd.)
15:20
CONFERENCE HALL A + B
Meet the Histochemical Society
Session Chairs: Liliana Schaefer
15:20‑15:45
Margarida Barroso, Albany Medical College, USA
Multiscale Imaging of Receptor Transport: Super-Resolution To Optical Imaging
15:45‑16:10
Joseph L. Cheatwood, SIU School of Medicine, USA
Immunohistochemistry as a Tool for Student-led and Collaborative Neuroscience Research
16:10‑16:35
A. Sally Davis, Kansas State University, USA
Insights about Rift Valley Fever gleaned from formalin-fixed, paraffin-embedded tissues
19:00 – 22:00
BEER PARTY at the Strahov Monastery Brewery
Supported by HPST, s.r.o.
Social program & Networking (free for all ICHC participants)
09:00
CONFERENCE HALL A
Sample processing, probes, histopathology and quantitative approaches (PART 1)
Session Chair: Vlada Filimonenko
09:00‑09:25
Christian Mühlfeld, Hannover Medical School, Germany (INVITED)
Robert Feulgen Prize Awardee of 2020
3D methods of analyzing the pulmonary vasculature and their application to studies of lung development
09:25‑09:40
František Kitzberger, Biology Centre, Czech. Acad. Sci., Czech Republic
Powerful method for simultaneous visualization and differentiation of Au nanoparticles on both sides of the ultrathin sections using low-energy STEM and BSE in HRSEM
09:40‑09:55
Peter Hoboth, Institute of Molecular Genetics, Czech Republic
Quantitative super-resolution microscopy reveals the spatial organization of the gene expression compartments in human cells and tissues
09:55‑10:10
Claudio Casali, Università degli Studi di Pavia, Italy
Oxidative DNA damage on the Cell Nucleus: an Ultrastructural and Super-Resolution Approach for Epigenetics Insights
09:00
CONFERENCE HALL B
Cell physiology, immunology and infectious diseases
Session Chair: Luca M. Neri
09:00‑09:25
Lucio Cocco, University of Bologna, Italy (INVITED)
Inositide-specific phospholipase C (PI-PLC) β1 signalling and location: a thirty five years tale
09:25‑09:50
Luca M. Neri, University of Ferrara, Italy (INVITED)
Microplastic uptake within human cells is time and dose dependent and displays a specific intracellular distribution
09:50‑10:15
Carolina Simioni, University of Ferrara, Italy (INVITED)
Assessment of the morphological tissue features of placental and vascular samples after SARS-CoV-2 infection
10:15‑10:35
Joanna Nowak, Medical University of Gdansk, Poland
VDR, RXR, and PDIA3 in vitamin D functioning
10:30
Coffee break
11:00
CONFERENCE HALL A + B
PLENARY LECTURE | Session Chair: Serap Arbak
Alev Erisir, University of Virginia, USA
Serial EM Image Reconstructions in Studying Selectivity and Network Properties of Synaptic Inputs
12:00
Lunch break
13:00
CONFERENCE HALL A + B
PLENARY LECTURE | Session Chair: Toyoshi Fujimoto
Acta Histochemica et Cytochemica lecture
Robert Y. Osamura, Nippon Koukan Hospital, Japan
Fifty enchanted years of my life with histochemistry
13:45
SEMINAR ROOM CRACOW I+II
POSTER SESSION: list of posters
15:15
Coffee break
15:45
CONFERENCE HALL A + B
Immunocytochemistry workshop: ‘Just Do It Right’
Session Chair: Charles Frevert
15:45‑16:10
(remote)
Stephen Hewitt, National Cancer Institute, USA
Pre-analytic Variables: Addressing Biology and Reproducibility in Histopathology
16:10‑16:35
Frank Peale, Genentech, Inc., USA
Synthetic Antigen Gels: Quantifiable Controls for Immunohistochemistry
16:35‑17:00
Charles Frevert, University of Washington, USA
Morphometric Analysis of Healthy and Diseased Tissues using Immunohistochemistry, In Situ Hybridization, and Quantitative Digital Pathology (QDP)
19:00
GALA DINNER at the Municipal House
Social program & Networking (with fee upon reservation)
09:00
CONFERENCE
HALL A
Neuroscience
Session Chairs: Bozena Kaminska,
Lucio Cocco
09:00‑09:25
Michal Stawarski, University of Basel, Switzerland (INVITED)
Functional characterization of monoallelic de novo GABAB receptor
variants identified in neurological and psychiatric disorders
09:25‑09:50
Monika Bijata, Nencki Institute of Experimental Biology, PAS, Poland (INVITED)
Activation of the 5-HT7 receptor and MMP-9 signaling module in the hippocampal
CA1 region is necessary for the development of depressive-like behavior
09:50‑10:15
Diego Clemente, Hospital Nacional de Parapléjicos, Spain (INVITED)
Morphofunctional characterization of Myeloid-Derived Suppressor Cells in
multiple sclerosis: from blood to brain
10:15‑10:30
Stefano Ratti, University of Bologna, Italy
The intriguing role of astrocytes in Autosomal-dominant leukodystrophy (ADLD)
10:30‑10:45
Esra Nur Yigit, İstanbul Medipol University, Turkey
Correlative Light and Electron Microscopy of Mutant Huntingtin Aggregate
Formation and its Relationship with Subcellular Components
09:00
CONFERENCE
HALL B
Sample processing, probes,
histopathology and quantitative
approaches (PART 2)
Session Chairs: Romina Mancinelli
09:00‑09:30
(remote)
Takehiko Koji, Nagasaki University Grad. Sch. of Biomed. Sci., Japan (INVITED)
Histochemistry and Cell Biology (HCB) Lecture
Differentiation-Dependent Changes in Epigenetic Factors in Mouse Spermatogenesis
and Their Possible Relations to Germ Cell Apoptosis
09:30‑09:45
Katherine Paine, University of York, United Kingdom
Young Histochemist Awardee
Using microscopy to understand molecular trafficking of cell surface proteins
09:45‑10:00
Dmitrii Atiakshin, Peoples’ Friendship University of Russia, Russian Federation
Combined histochemical approach in assessing naphthol AS-D chloroacetate
esterase activity of mas
10:00‑10:15
Elisa Boschetti, University of Bologna, Italy
Altered microvasculature in idiopathic chronic intestinal pseudo-obstruction:
a quantitative morphometric and molecular analysis
10:15‑10:30
Vlada Filimonenko, Institute of Molecular Genetics, Czech Republic
Faster Cryo TEM lamella preparation using optimised conditions and advanced workflows
10:30‑10:45
Ludovica Ceci, Sapienza University of Rome, Italy
Long term effects of melatonin on cholestatic murine model
10:45
Coffee break
11:15
CONFERENCE
HALL A + B
COMPANY PRESENTATION | Session Chair: TBA
TESCAN
11:45
CONFERENCE
HALL A + B
Stem cell biology and regenerative
medicine & Cell differentiation
and developmental biology
Session Chair: Christian Schöfer
11:45‑12:10
Roberta Di Pietro, G. d’Annunzio University of Chieti-Pescara, Italy (INVITED)
Mapping of the Human Amniotic Membrane: Identification of Functionally Distinct
Subregions Useful for Regenerative Medicine Applications
12:10‑12:35
Sercin Karahuseyinoglu, Koc University School of Medicine, Turkey (INVITED)
Application of Genome Edition Technology in Reproductive and Developmental
Biology
12:35‑12:50
Alexander Dulebo, Bruker Nano GmBH, JPK BioAFM, Germany
Application of Large Area Mapping AFM for Automated Structural and Mechanical
Analysis of Developing Cells and Tissues
13:50
CONFERENCE
HALL A + B
KEYNOTE LECTURE | Session Chair: Pavel Hozak
David Glick Award lecture
Stefan Hell, Max Planck Institute for Biophysical Chemistry, Germany
MINFLUX nanoscopy and related matters
14:50
CONFERENCE
HALL A + B
YOUNG HISTOCHEMIST
AWARD SESSION
Session Chair: Roberta Di Pietro
14:50‑15:05
Maria Vittoria Marvi, University of Bologna, Italy
Phospholipases: novel potential modulators of aggressiveness in Glioblastoma
15:05‑15:20
(remote)
Mohammed Khurshed, Amsterdam UMC, Netherlands
Metabolic vulnerabilities in IDH1/2-mutated solid tumours as basis for a phase
IB/II clinical trial using metformin and chloroquine
15:20‑15:35
Katerina Cizkova, Palacky University in Olomouc, Czech Republic
Effect of Heat-induced Antigen Retrieval in Immunocytochemical Staining in
Alcohol-fixed Cell Samples: Subcellular Localization of Antigens Matters
15:35‑15:50
Wioletta Zielinska, Nicolaus Copernicus University, Poland
TRPM2 calcium channel as a target for the prevention of endothelial dysfunction caused
by oxidative stress
15:50‑16:05
Selenay Furat Rencber, Kocaeli University, Turkey
Endoplasmic Reticulum (ER) Stress of Endometrial Stem Cells in Endometriosis
16:05
CONFERENCE HALL A + B
CLOSING CEREMONY
ICHC 2022 conclusion by the IFSHC representatives
List of Posters
P-1 | Aleksic | Marija | Peroxisomal Biogenesis In WAT Depots Of Hypothyroid Rats |
P-2 | Antiga | Ludovica | The role of phosphatidylinositol 4,5-bisphosphate in transcription |
P-3 | Arbak | Serap | Microscopical Evaluation of the Healing Effect of Apocynin on Monosodium Glutamate -induced Liver damage in rats. |
P-4 | Aydin | Mehmet Şerif | Migration and Differentiation of Breast Milk Stem Cells into the Brain of Mouse Offspring After Cerebral Ischemia |
P-5 | Balaban | Can | The PIP2 effector protein MPRIP regulates RNA Polymerase II condensation and transcription |
P-6 | Bijata | Krystian Robert | New chapter in a long story – The role of monoamine oxidases palmitoylation during chronic stress |
P-7 | Brun | Paola | Bone Morphogenic Proteins expression in the lower airways of stable COPD patients |
P-8 | Cizkova | Dana | Immunohistochemical detection of nestin in the rat and human hypertrophic myocardium |
P-9 | Demirci | Hasan | Nephrotoxic side effects of the calcineurin inhibitors, cyclosporin A and tacrolimus, affect glomeruli and tubulointerstitium differentially in chronic experimental settings |
P-10 | Di Angelo | Luca | Ultrastructural biomarkers of human pancreas adenocarcinoma (PDAC): a pilot study. |
P-11 | Dilber | Yağmur | The Effect of Egcg Anti-Apoptotic Pathway On Ischemia-Reperfusion Damage in Rat Testicular Tissue |
P-12 | Filimonenko | Vlada | IMG Electron Microscopy Core Facility |
P-13 | Golic | Igor | Missing link between UCP1 harlequinism and glycogen deposition in brown adipocytes induced by insulin |
P-14 | Grabowska | Marta | The effects of different immunosuppressive therapies on rat testis morphology and testicular immunoexpression of aquaporins |
P-15 | Higuchi | Naoko | Histopathological cytochemical analysis of Pseudomonas aeruginosa infection in mice on the mechanism of action of novel humanized monoclonal antibody ‘h1F3’ against PcrV protein |
P-16 | Hosoki | Ryosuke | Noninvasive manipulation of actin polymerization in living cells: experimental applications of terahertz wave irradiation and fluorocarbon solvents |
P-17 | Jabłońska | Karolina | Prolactin-induced protein (PIP) increases the sensitivity of breast cancer cells to drug-induced apoptosis |
P-18 | Janikiewicz | Paweł | Distribution and neurochemical characterization of dorsal root ganglia (DRG) neurons containing phoenixin (PNX) and supplying the porcine urinary bladder. |
P-19 | Kiezun | Jacek | The expression of chemerin in stromal/immune cells in colorectal cancer |
P-20 | Kmieć | Zbigniew | Analysis of SHH pathway elements and VEGFA expression in kidney renal clear cell carcinoma |
P-21 | Kolanko | Emanuel | Optimization of the intrasplenic human amniotic epithelial cell injection via the subcutaneous splenic port in murine model |
P-22 | Lewczuk | Bogdan | Volume light and electron microscopy in studies of embryonic development of the avian pineal gland |
P-23 | Melichar | Adolf | Tinnitus-related changes in GABAB receptor expression in the mouse dorsal cochlear nucleus |
P-24 | Miladinović | Ana | Localization and molecular mechanisms of nuclear functions of phosphoinositide-3,4-bisphosphate |
P-25 | Mlynarczuk-Bialy | Izabela | Surviving entotic Cell in Cell (CIC) in breast cancer as potential prognostic marker |
P-26 | Nicu | Alina-Teodora | DNMT Inhibitors Modulate Histone Methylation: Epigenetic Crosstalk Between H3K4me3 And DNA Methylation During Sperm Differentiation |
P-27 | Nowińska | Katarzyna | Role of Irisin/FNDC5, ERRα and PGC-1α expression in NSCLC |
P-28 | Opelka | Jakub | An Efficient Method for Quantifying the Degree of Neurodegeneration in an Insect Brain |
P-29 | Podlahová | Šárka | Effect of the venom of Habrobracon hebetor on Ephestia kuehniella and Drosophila melanogaster muscles |
P-30 | Schrader | Michael | New insights into peroxisome dynamics: PEX11β and FIS1 cooperate in peroxisome division independent of Mitochondrial Fission Factor |
P-31 | Sezer | Ayşe Server | Comparative Analysis of Intrinsic Regenerative Patterns of Axolotl and Mouse Sensory Neurons |
P-32 | Sezer | Zehra | The role of mitochondrial dynamic during endometrial decidualization and gestation |
P-33 | Skopalík | Josef | Endothelial barrier and liposomes diffusion under different biophysical stimulation |
P-34 | Szyryńska | Natalia | Towards the best protocol for multiscale ultrastructural imaging of large sample areas in the pineal research |
P-35 | Tok | Olgu Enis | A novel autofluorescence quenching technique on testis tissue. |
P-36 | Tok | Olgu Enis | A Novel Cell Culture Device and Methodology to Prevent the Damage and Loss of Sensitive Cells Caused by Centrifugation |
P-37 | Torga | Taavi | The effect of different antigen retrieval methods on the immunohistochemical staining of cartilage intermediate layer protein 2 (CILP-2) |
P-38 | Ucci | Maria Assunta | The inflammatory milieu in natural ovarian aging: is it possible to counteract it? |
